Comparative Anticancer Efficacy Analysis of T. rufonigra, C. oblongus, A. gracilipes and Camponotus sp. of Ants: An in vitro Study

ABSTRACT: Insects and their products have been linked to medical cures from age old now. Among all the other Insects, Ants of order Hymenoptera possess a wide range bioactive compounds that shown potent anticancer properties. In similar fashion, present study investigates in vitro antitumor effects Bengaluru-based ant extracts. Different species were collected various locations Bengaluru identified as Tetraponera rufonigra, Camponotus oblongus, Anoplolepsis gracilipes, species. Further, A 3-(4, 5-dimethylthiazolyl2)-2, 5-diphenyltetrazolium bromide (MTT) assay was performed on hepatocellular carcinoma HepG2 after extracting metabolites 95% EtOH. The potential effect again confirmed by Trypan blue cell staining using (hepatocellular carcinoma) MCF7 (human breast adenocarcinoma) line. apoptotic induction measured Caspase-3 activity different tests investigate chemical composition All crude extracts ants increase caspase-3 enzyme rufonigra extract while gracilipes human cancer line makes them good candidate for further purification characterization. T. presence tested chemicals like alkaloids, flavonoids, reducing sugars, phenols, steroids amino acids.